Regional chromosome mapping of human collagen genes alpha 2(I) and alpha 1(I) (COLIA2 and COLIA1)
- 1 April 1985
- journal article
- research article
- Published by Springer Nature in Human Genetics
- Vol. 69 (4), 304-308
- https://doi.org/10.1007/bf00291646
Abstract
For the assignment of the genes for the pro-α2(I) (COLIA2) and the pro-α1(I) (COLIA1) collagens, cDNA and genomic DNA probes were used in in situ hybridization experiments on human prometaphase chromosomes. An improved staining method is reported for the simultaneous identification of chromosomes and the autoradiographic grains after the hybridization procedures. With this procedure more cells with higher resolution could be used for the assignment of genes by in situ hybridization. Statistical analysis of the grains located on respectively 660 and 302 metaphases using pro-α2(I) and proα1(I) DNA probes, confirmed the assignment of these genes to human chromosomes 7 and 17. Analysis of the grain distribution on prometaphase chromosomes showed that the location of the proα2(I) collagen gene is in the region 7q21.3–22.1. The location of the pro-α1(I) collagen gene was found to be in band 17q21.31–2005.Keywords
This publication has 19 references indexed in Scilit:
- Report of the committee on the genetic constitution of chromosomes 13, 14, 15, 16 and 17Cytogenetic and Genome Research, 1984
- Report of the committee on the genetic constitution of chromosomes 7, 8 and 9Cytogenetic and Genome Research, 1984
- Confirmation that the Type I collagen gene on chromosome 17 is COL1A1 (α1(I)), using a human genomic probeAnnals of Human Genetics, 1984
- Localization of the human 2(I) collagen gene (COL1A2) to chromosome 7q22Cytogenetic and Genome Research, 1983
- Regional localization of the human Rα2(I) collagen gene on chromosome 7 by molecular hybridizationCytogenetic and Genome Research, 1983
- Human type I procollagen genes are located on different chromosomes.Proceedings of the National Academy of Sciences, 1982
- Regional chromosome mapping of the human skin type I procollagen gene using adenovirus 12-fragmentation of human-mouse somatic cell hybridsCytogenetic and Genome Research, 1980
- Assignment of a type I collagen structural gene to human chromosome 7Nature, 1978
- A high resolution in situ hybridization technique for the direct visualization of labeled G-banded early metaphase and prophase chromosomesCytogenetic and Genome Research, 1978
- Genetics of the connective tissue proteins: Assignment of the gene for human type I procollagen to chromosome 17 by analysis of cell hybrids and microcell hybridsProceedings of the National Academy of Sciences, 1977