Surface Properties of Class II Hydrophobins from Trichoderma reesei and Influence on Bubble Stability
- 20 June 2007
- journal article
- research article
- Published by American Chemical Society (ACS) in Langmuir
- Vol. 23 (15), 7995-8002
- https://doi.org/10.1021/la700451g
Abstract
We report the remarkable surface behavior of class II hydrophobin proteins HFBI and HFBII from Trichoderma reesei and the resulting effect that these proteins have on the stability of air bubbles to the process of disproportionation. The surface properties were studied using surface tensiometry and surface shear rheology. Surface tensiometry data show that hydrophobins are very surface active proteins, reducing the surface tension to approximately 30 mN m-1. The rate at which the hydrophobins adsorb at the surface may also be related to the self-assembly behavior in aqueous solution. We further show that hydrophobins form air/water surfaces with high elasticity, the magnitude of which is well in excess of that of surface layers formed by other common proteins used as foam or emulsion stabilizers. The measured surface properties translate to the stability of bubbles with adsorbed hydrophobin, and in this study, we demonstrate the ability of hydrophobin to have a dramatic effect on the rate of disproportionation in some simple bubble dissolution studies.Keywords
This publication has 23 references indexed in Scilit:
- Interaction and Comparison of a Class I Hydrophobin from Schizophyllum commune and Class II Hydrophobins from Trichoderma reeseiBiomacromolecules, 2006
- Hydrophobins: the protein-amphiphiles of filamentous fungiFEMS Microbiology Reviews, 2005
- Adsorption of hydrophobin proteins at hydrophobic and hydrophilic interfacesColloids and Surfaces B: Biointerfaces, 2005
- Hydrophobins: proteins with potentialCurrent Opinion in Biotechnology, 2005
- Foam stability: proteins and nanoparticlesCurrent Opinion in Colloid & Interface Science, 2004
- Structural Hierarchy in Molecular Films of Two Class II HydrophobinsBiochemistry, 2003
- The Hydrophobins HFBI and HFBII from Trichoderma reesei Showing Efficient Interactions with Nonionic Surfactants in Aqueous Two-Phase SystemsBiomacromolecules, 2001
- Effect of Bulk and Interfacial Rheological Properties on Bubble DissolutionJournal of Colloid and Interface Science, 2001
- Interfaces: their role in foam and emulsion behaviourCurrent Opinion in Colloid & Interface Science, 2000
- Structural Characterization of the Hydrophobin SC3, as a Monomer and after Self-Assembly at Hydrophobic/Hydrophilic InterfacesBiophysical Journal, 1998