Synthesis of radioactive dolichol from [4S-3H]-mevalonate in the regenerating rat liver
- 15 February 1974
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 138 (2), 277-280
- https://doi.org/10.1042/bj1380277
Abstract
The dolichol of rat liver was labelled by injecting [4S-3H]mevalonate, the precursor of cis-isoprene residues, into partially hepatectomized animals. The optimum conditions for labelling the dolichol were to inject the animals with radioactive mevalonate 48h after hepatectomy and to kill them 12h later. The concentration of radioactive dolichol was five times as great in regenerating rat liver as in normal liver. The highest concentration of radioactive dolichol was found in the crude mitochondrial and nuclear-debris fractions of the cell. The crude microsomal fractions also contained radioactive dolichol, but at a lower concentration.Keywords
This publication has 14 references indexed in Scilit:
- The involvement of endogenous dolichol in the formation of lipid-linked precursors of glycoprotein in rat liverBiochemical Journal, 1974
- The characterization and stereochemistry of biosynthesis of dolichols in rat liverBiochemical Journal, 1970
- Isolation of chick intestinal nuclei. Effect of vitamin D3 on nuclear metabolismBiochemical Journal, 1969
- Isolation of rough and smooth microsomes from rat liver by means of a commercially available centrifugeAnalytical Biochemistry, 1969
- Intracellular distribution of the free and esterified forms of dolichol in pig liverArchives of Biochemistry and Biophysics, 1968
- The structure of bactoprenol, a lipid formed by lactobacilli from mevalonic acidBiochemical Journal, 1966
- In vivo incorporation of [2-14C]-mevalonate into dolichol of rabbit and pig liverArchives of Biochemistry and Biophysics, 1966
- Studies on the biosynthesis of cholesterol XIX. Steric course of hydrogen eliminations and of C—C bond formations in squalene biosynthesisProceedings of the Royal Society of London. B. Biological Sciences, 1966
- Regeneration of Mammalian LiverInternational Review of Cytology, 1963
- The intracellular distribution of dolichol in pig liverBiochemical Journal, 1962