Time and cell systems as variables in fusion experiments with polyethylene glycol
- 1 January 1978
- journal article
- research article
- Published by Springer Nature in Human Genetics
- Vol. 42 (3), 319-322
- https://doi.org/10.1007/bf00291313
Abstract
Cell hybridization was done between a monolayer of B14-150 Chinese hamster cells and a suspension of either mouse leukemia cells or normal human lymphocytes. Cell contact was obtained by centrifugation of the suspension cells onto the monolayer cells in a culture plate. Cell fusion was done by means of polyethylene glycol (PEG). The optimum time for PEG exposure as well as the yield of hybrid cells differed markedly with the different combinations.This publication has 15 references indexed in Scilit:
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