The pectic enzymes of Aspergillus niger. 2. Endopolygalacturonase
- 1 April 1961
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 79 (1), 57-64
- https://doi.org/10.1042/bj0790057
Abstract
Endo-polygalacturonase has been separated from the culture fluid of Aspergillus niger with a purification factor of 400-fold and a recovery of 34%. The preparation obtained was homogeneous on paper electrophoresis at pH 7-2 and pH 8-0, and of moderate stability with an optimum for stability of about pH 6. The enzyme rapidly lowered the viscosity of a solution of pectic acid and released reducing groups from it according to a random pattern of hydrolysis, but the digestion was not complete. Pectin underwent only a very slight degradation, sufficient to show a fall in viscosity but with little release of reducing groups. Tetragalact-uronic acid was rapidly split to tri- and mono-galacturonic acid, and trigalacturonic acid was slowly split to di- and mono-galacturonic acid, but the digalacturonic acid was not attacked.Keywords
This publication has 12 references indexed in Scilit:
- The pectic enzymes of Aspergillus niger. 1. The production of active mixtures of pectic enzymesBiochemical Journal, 1961
- On the Mechanism of Action of Yeast Endo-Polygalacturonase on Oligogalacturonides and Their Reduced and Oxidized DerivativesJournal of Biological Chemistry, 1959
- THE UNIENZYMATIC NATURE OF YEAST POLYGALACTURONASEJournal of Biological Chemistry, 1956
- HYDROLYSIS OF THE OLIGOGALACTURONIDES AND PECTIC ACID BY YEAST POLYGALACTURONASEJournal of Biological Chemistry, 1954
- End products and mechanism of hydrolysis of pectin and pectic acid by yeast polygalacturonase (YPG)Archives of Biochemistry and Biophysics, 1954
- Action of pectic enzymes on oligogalacturonic acids and some of their derivativesArchives of Biochemistry and Biophysics, 1954
- BREAKDOWN OF PECTIC SUBSTANCES BY A NEW ENZYME FROM NEUROSPORAJournal of Biological Chemistry, 1952
- The pectic enzymes of the fungus Byssochlamys fulvaBiochemical Journal, 1951
- ELECTROPHORESIS OF PROTEINS ON FILTER PAPERThe Journal of general physiology, 1951
- Detection of Sugars on Paper ChromatogramsNature, 1950