Tropane alkaloid production in cultured cells of Duboisia leichhardtii
- 1 October 1984
- journal article
- research article
- Published by Springer Nature in Plant Cell Reports
- Vol. 3 (5), 186-188
- https://doi.org/10.1007/bf00270196
Abstract
Calluses were derived from explants of aerial parts of a Duboisia leichhardtii F. Muell. plant. After 3 months of culture we detected small amounts of hyoscyamine and scopolamine in the unorganized calluses, but the amounts decreased until they were undetectable with successive subculture. When shoots were produced from the unorganized calluses, the ability to convert hyoscyamine added into media to scopolamine appeared; when roots were initiated from the shoot-organizing calluses, hyoscyamine, scoporamine, and nicotine were synthesized. Cultured adventitious roots produced these alkaloids and converted exogenous hyoscyamine to scopolamine.Keywords
This publication has 11 references indexed in Scilit:
- Scopolamine Production in Suspension Cultures and Redifferentiated Roots ofHyoscyamus nigerPlanta Medica, 1983
- Studies on Duboisia Species. V. Alkaloids in Cultured Cells of Duboisia leichhardtiiYAKUGAKU ZASSHI, 1980
- Organization and metabolism of exogenous hyoscyamine in tissue cultures of a Duboisia hybridThe Science of Nature, 1979
- Un gradient metabolique: Rapport scopolamine/hyoscyamine dans les feuilles du Duboisia myoporoides en fonction de leur niveau d'insertion et du stade de croissancePhytochemistry, 1979
- Morphogenetic and biosynthetic studies on tissue cultures of Atropa belladonna L.Plant Science Letters, 1978
- ALKALOIDE IM KALLUS VON DUBOISIA MYOPOROIDESPlanta Medica, 1975
- Formation of acetyltropine in Datura callus culturesPhytochemistry, 1973
- (−)-Hyoscyamine in Duboisia hopwoodiiPhytochemistry, 1971
- Nutrient requirements of suspension cultures of soybean root cellsExperimental Cell Research, 1968
- Organic Growth Factor Requirements of Tobacco Tissue CulturesPhysiologia Plantarum, 1965