The assay of gastrin using the perfused rat stomach
Open Access
- 1 January 1970
- journal article
- Published by Wiley in British Journal of Pharmacology
- Vol. 38 (1), 206-213
- https://doi.org/10.1111/j.1476-5381.1970.tb10349.x
Abstract
1 A method is described for the bio-assay of gastric secretagogues using the perfused rat stomach in which the acid output can be readily and accurately quantified. 2 This technique increases the sensitivity of the perfused rat stomach so that 10–20 ng of synthetic human gastrin I can be detected. 3 It is possible to assay secretagogues, relative to a standard with fiducial limits of less than ±30%. 4 The use of this system is illustrated by assays comparing synthetic human gastrin I with pentagastrin, pure hog gastrin and gastrin added to plasma.Keywords
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