Transformation of peas
- 1 January 1993
- journal article
- Published by Springer Nature in Plant Cell Reports
- Vol. 12 (3), 180-183
- https://doi.org/10.1007/bf00239102
Abstract
The lateral cotyledonary meristems present in germinating seed were inoculated with a non-oncogenic strain of A. tumefaciens carrying a gene conferring kanamycin resistance as a selectable marker and a β-glucuronidase sequence as a reporter gene. Kanamycin resistant plants were derived from the meristems and shown to be transformed on the basis of Southern blots, polymerase chain reaction analysis and tests for β-glucuronidase activity. The plants were fertile and tests of their progeny confirmed the transmission of integrated sequences through a sexual generation. This transformation method has the merit of an unlimited supply of material for inoculation and a relatively short time scale from inoculation to the production of rooted plants.Keywords
This publication has 8 references indexed in Scilit:
- Inheritance of a bacterial hygromycin phosphotransferase gene in the progeny of primary transgenic pea plantsTheoretical and Applied Genetics, 1992
- The effect of T-DNA copy number, position and methylation on reporter gene expression in tobacco transformantsPlant Molecular Biology, 1990
- Agrobacterium tumefaciens-mediated transformation of Pisum sativum L. using binary and cointegrate vectorsPlant Cell Reports, 1990
- Production of transgenic pea (Pisum sativum L.) plants by Agrobacterium tumefaciens — mediated gene transferTheoretical and Applied Genetics, 1990
- Epigenetic changes in the expression of the maize A1 gene inPetunia hybrida: Role of numbers of integrated gene copies and state of methylationMolecular Genetics and Genomics, 1990
- Transformation of Brassica napus with Agrobacterium tumefaciens based vectorsPlant Cell Reports, 1987
- beta-Glucuronidase from Escherichia coli as a gene-fusion marker.Proceedings of the National Academy of Sciences, 1986
- A technique for radiolabeling DNA restriction endonuclease fragments to high specific activityAnalytical Biochemistry, 1984