Myelin‐Associated Glycoprotein and Other Proteins in Trembler Mice
- 1 March 1985
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 44 (3), 793-797
- https://doi.org/10.1111/j.1471-4159.1985.tb12885.x
Abstract
The myelin-associated glycoprotein (MAG) and other myelin proteins were quantitated in homogenates of whole sciatic nerve from adult and 20-day-old Trembler mice. In the nerves of adult mice, the concentration of MAG was increased from 1.1 ng/.mu.g of total protein in the controls to 1.4 ng/.mu.g protein in the Tremblers. The concentrations of P0 glycoprotein and myelin basic proteins [MBP] were reduced to 27% and 20% of control levels, respectively. Immunoblots demonstrated that P2 was also greatly reduced in the Trembler nerves. The specific activity of 2'',3''-cyclic nucleotide 3''-phosphodiesterase (CNP) was 65% of the control level. Immunoblot analysis showed that MAG had a higher than normal apparent MW in the sciatic nerves of the Trembler mice, but its apparent MW was normal in the brains of these mutants. In 20-day-old Tremblers, the P0 and MBP were reduced slightly less to .apprx. 40% of the level in the nerves of age-matched controls. CNP and MAG levels were not significantly different from those in controls and MAG exhibited a shift toward higher apparent MW similar to that in the adults. The maintenance of high MAG levels despite the severe deficit of myelin, as reflected by the decrease of the major myelin proteins, is consistent with the immunocytochemical localization of MAG in periaxonal Schwann cell membranes, Schmidt-Lantermann incisures, lateral loops and the outer mesaxon and its absence from compact myelin. The abnormal form of MAG in the peripheral nervous system (PNS) of the Trembler mice may contribute to the pathology in this mutant.Keywords
This publication has 28 references indexed in Scilit:
- Lounging in a lysosome: the intracellular lifestyle of Coxiella burnetiiCellular Microbiology, 2007
- Development and Applications of a Solid‐Phase Radioimmunoassay for the P0 Protein of Peripheral MyelinJournal of Neurochemistry, 1983
- Alteration of 5′‐Nucleotidase and Na+, K+‐ATPase in Central and Peripheral Nervous Tissue from Dysmyelinating Mutants (jimpy, quaking, Trembler, shiverer, and mld). Comparison with CNPase in the Developing Sciatic Nerve from TremblerJournal of Neurochemistry, 1982
- Behavior of Schwann cells from Trembler mouse unmyelinated fibers transplanted into myelinated nervesExperimental Neurology, 1981
- SCHWANN CELL MULTIPLICATION IN TREMBLER MICENeuropathology and Applied Neurobiology, 1981
- Evidence for the Association of 2′,3′‐Cyclic‐Nucleotide 3′‐Phosphodiesterase with Myelin‐Related Membranes in Peripheral Nervous SystemJournal of Neurochemistry, 1980
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- Developmental change in a myelin-associated glycoprotein: a comparative study in rodentsDevelopmental Biology, 1974
- ANOMALIES OF MYELIN‐ASSOCIATED GLYCOPROTEINS IN‘QUAKING MICEJournal of Neurochemistry, 1974
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970