Über den Gehalt des Blutes an Cerebrosiden

Abstract

One cc. of whole blood. serum, plasma or washed and hemolyzed red cells, was placed in a 100 cc. Erlenmeyer flask containing 15-20 cc. 96% ethyl alc. The mixture was heated to boiling on the H2O bath, filtered and the filtrate evaporated to dryness. The residue was extracted with 4 cc. of CHC13 containing 20% C2H5OH. followed by 3 cc. of the C2H5OH-CHC13, and the solvent shaken with 10 cc. 2% aqueous trichloracetic acid soln. The cerebroside was precipitated and formed a thin layer between the 2 phases. The trichloracetic acid layer was removed, the remainder of the solvents evaporated to dryness, the cerebrosides dissolved in 0.5-1.0 cc. C2H5OH, and the cerebrosides detd. according to the method previously descr. (Hoppe-Seyler''s Zeitschr. physiol. Chem., 268: 163-170). The blood of 23 normal adults was examined. Cerebroside was not found in the plasma or serum. The cerebroside conc. of the washed red cells varied from 25.4-45.4 mg.%, the avg. being 34.8 mg.%. The cerebroside conc. of whole blood was about 18.7 mg.%. The cerebroside conc. in the leucocytes was about 210 mg.%.