Cyclic GMP as the second messenger in helper cell requirement for gamma-interferon production.
- 1 December 1982
- journal article
- research article
- Published by The American Association of Immunologists in The Journal of Immunology
- Vol. 129 (6), 2570-2572
- https://doi.org/10.4049/jimmunol.129.6.2570
Abstract
Cyclic GMP and activators (acetylcholine, E. coli heat-stable toxin) of guanylate cyclase were capable of completely replacing the helper cell or interleukin 2 requirement for gamma-interferon (IFN gamma) production by Lyt-1-,2+ cells from C57BL/6 mouse spleen cells. The cyclic GMP help was independent of DNA synthesis or proliferation in the IFN gamma-producing cells, because cyclic GMP reversed mitomycin C blockage of IFN gamma production but did not reverse the inhibition of DNA synthesis. Thus, the findings presented here are unrelated to the question of the second messenger role of cyclic GMP in the activation of lymphocytes for DNA synthesis and cellular proliferation. The cyclic GMP help for IFN gamma production was antagonized by cyclic AMP and inducers (isoproterenol) of adenylate cyclase.This publication has 12 references indexed in Scilit:
- Phorbol ester replacement of helper cell and interleukin 2 requirements in gamma interferon productionInfection and Immunity, 1982
- Interleukin 2 regulates immune interferon (IFN gamma) production by normal and suppressor cell cultures.The Journal of Immunology, 1982
- Cellular Regulation of Gamma Interferon Production: Lyt Phenotype of the Suppressor CellInfection and Immunity, 1982
- Activation of lymphocytes by brominated nucleoside and cyclic nucleotide analogues: implications for the "second messenger" function of cyclic GMP.Proceedings of the National Academy of Sciences, 1981
- Phytohemagglutinin stimulation of guanylate cyclase in human lymphocytes.Journal of Biological Chemistry, 1981
- Cellular regulation of immune interferon productionAntiviral Research, 1981
- Classification of interferons with antibody to immune interferonCellular Immunology, 1980
- Heat-stable enterotoxin of Escherichia coli: in vitro effects on guanylate cyclase activity, cyclic GMP concentration, and ion transport in small intestine.Proceedings of the National Academy of Sciences, 1978
- Evidence for Cyclic GMP and Calcium Mediation of Lymphocyte Activation by MitogensThe Journal of Immunology, 1977
- Separation of mitogen-induced suppressor and helper cell activities during inhibition of interferon production by cyclic AMPCellular Immunology, 1977