Electrophoretic Fractionation of B12-Binders in Gastric Juice from Patients with Pernicious Anemia and from Controls.

Abstract

Radiovitamin B12 was added in vitro to human gastric juice. Paper electrophoresis was done with ultrafiltrated juice, and the strips were autoradiographed. Two B12-binders were found in acid gastric juice as well as in gastric juice from patients with histamine fast achlorhydria and normal Schilling test. The slower migrating B12-binder was absent in gastric juice from patients with pernicious anemia; here only the faster migrating B12-binder was found. The investigation lends support to the view that the slower migrating B12-binder in electrophoresis of gastric juice is related to intrinsic factor. It is suggested that the method may be used for in vitro assay of intrinsic factor.