Evaluation of Detergent Analysis in Estimating Nutritional Value of Browse

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Abstract
The efficiency of carbohydrate and protein extraction from browse by detergent solutions was investigated. Standard and sequential acid-detergent [AD] extractions removed virtually all of the hemicellulose, total soluble sugars, water-soluble polysaccharides and pectin. Sequential extraction of browse with neutral and acid detergents removed more of the protein than either neutral detergent [ND] or separate acid detergent, while quantitatively retaining the cellulose. Neutral detergent with sodium sulfite removed an average of 22% of the hemicellulose, and probably solubilized portions of the true lignin and cutin. Consequently, sodium sulfite should not be used in the neutral-detergent procedure, ND-AD sequential analyses should be used, and hemicellulose must be determined colorimetrically if a precise determination is necessary.