A Conditional Lethal Mutant of Escherichia coli K12 Defective in the 5′ → 3′ Exonuclease Associated with DNA Polymerase I
- 1 May 1974
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 71 (5), 2048-2051
- https://doi.org/10.1073/pnas.71.5.2048
Abstract
A mutant strain of E. coli, initially identified by an abnormally high frequency of recombination, has been found to be defective in the 5' --> 3' exonuclease associated with DNA polymerase I, but not in the polymerase activity. This defect is tolerated at 30 degrees , but is lethal at 43 degrees . Like other polymerase I mutants, the strain is unusually sensitive to methyl methanesulfonate and to ultraviolet irradiation; it is also unable to support the growth of phage lambda defective in general recombination, and shows a reduced rate of joining of 10S "Okazaki fragments." These results demonstrate that a functional DNA polymerase I is essential for normal growth and viability in E. coli K12.Keywords
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