Differential Liver Protein Expression during Schistosomiasis
- 1 February 2007
- journal article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 75 (2), 736-744
- https://doi.org/10.1128/iai.01048-06
Abstract
The arrival of eggs in the liver during Schistosoma mansoni infection initiates a protective granulomatous response; however, as the infection progresses, this response results in chronic liver fibrosis. To better understand the impact of schistosomiasis on liver function, we used a proteomic approach to identify proteins whose expression was significantly altered in schistosome-infected mice 8 weeks postinfection. Identification of differentially expressed proteins by mass fingerprinting revealed that schistosome infection markedly reduced the abundance of proteins associated with several normal liver functions (i.e., citric acid cycle, fatty acid cycle, and urea cycle), while proteins associated with stress responses, acute phase reactants, and structural components were all significantly more abundant. The expression patterns of several immunity-related proteins (peroxiredoxin 1, arginase 1, and galectin 1) suggested that different protein forms are associated with schistosome infection. These findings indicate that acute schistosomiasis has a significant impact on specific liver functions and, moreover, that the alterations in specific protein isoforms and upregulation of unique proteins may be valuable as new markers of disease.Keywords
This publication has 78 references indexed in Scilit:
- Galectin-1 is an inductor of pancreatic stellate cell activationCellular Signalling, 2005
- Overoxidation of peroxiredoxins as an immediate and sensitive marker of oxidative stress in HepG2 cells and its application to the redox effects induced by ischemia/reperfusion in human liverFree Radical Research, 2005
- Changes in expression and activity of glutathione S‐transferase in different organs of schistosoma haematobium‐infected hamsterJournal of Biochemical and Molecular Toxicology, 2003
- Induction of Peroxiredoxins in Transplanted Livers and Demonstration of TheirIn VitroCytoprotection ActivityAntioxidants and Redox Signaling, 2000
- Regulation of Genes for Inducible Nitric Oxide Synthase and Urea Cycle Enzymes in Rat Liver in Endotoxin ShockBiochemical and Biophysical Research Communications, 2000
- The Reticulocalbin Gene Maps to the WAGR Region in Human and to the Small Eye Harwell Deletion in MouseGenomics, 1997
- SCHISTOSOMIASIS: Pathophysiology, Diagnosis, and TreatmentGastroenterology Clinics of North America, 1996
- Overexpression of nm23-H1 and nm23-H2 genes in colorectal carcinomas and loss of nm23-H1 expression in advanced tumour stages.Gut, 1995
- Serum Procollagen Peptides and Collagen Type Vi for the Assessment of Activity and Degree of Hepatic Fibrosis in Schistosomiasis and Alcoholic Liver DiseaseHepatology, 1992
- Hepatic aldehyde dehydrogenase activity in liver diseases, with particular emphasis on alcoholic liver diseaseHepatology, 1989