Nuclear ribonucleoprotein particles from adenovirus infected Hela cells

Abstract

Heterogenous nuclear RNA-protein complexes (hnRNP) from adenovirus-2 (Ad-2) infected Hela cells contain most of the virus-specific RNA which is labeled in the nucleus during periods lasting from 45 seconds to 2 hours. Moreover, the percentage of RNA which is Ad-2 specific as monitored by filter hybridization increases progressively from early to late period where it accounts for as much as 50–60% of the labeled RNA. The Ad-2 sequences are found in heterogenous complexes sedimenting between 30 and 200 S the density of which in CsCl (p⊃1.39) as well as in metrizamide (p⊃1.29) seems to be the same as that of the bulk particles. A more detailed analysis with restriction fragments shows that all regions of the Ad-2 genome are represented in these particles.