Phosphorylation of elongation factor 1 in polyribosome fraction of rabbit reticulocytes
- 29 October 1984
- journal article
- Published by Wiley in FEBS Letters
- Vol. 176 (2), 401-405
- https://doi.org/10.1016/0014-5793(84)81206-5
Abstract
A single protein, Mr, ~ 50000, is shown to be phosphorylated during incubation of a mono- and polyribosome fraction of rabbit reticulocytes with [γ-32P]ATP at a low ionic strength. This protein has been identified as the elongation factor 1α (EF-1α). The phosphorylated EF-1α, in contrast to the unmodified factor, is not detected in complexes with mono- and polyribosomes. It is suggested that the phosphorylation of EF-lα can result in its decompartmentation from polyribosomes and thus affect the rate of protein synthesisKeywords
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