DNA ORGANIZATION IN THE MATURE SPERM OF SEVERAL ORTHOPTERA BY THE METHOD OF POLARIZED FLUORESCENCE MICROSCOPY
Open Access
- 1 August 1968
- journal article
- research article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 38 (2), 426-436
- https://doi.org/10.1083/jcb.38.2.426
Abstract
Mature sperm of Acheta domesticus, Acheta assimilis, Nemobius fasciatus, Nemobius confusus, Melanoplus femur-rubrum, Romalea microptera, Scudderia curvicauda, and Ceuthophilus nigricans were examined for DNA configuration by means of polarized fluorescence microscopy. In all cases, the results suggest that the DNA lies in unsupercoiled array, predominantly parallel to the elongate sperm head axes. Detailed calculations of the factors influencing the degree of polarization of the fluorescent emission from supercoiled DNA are contained in an appendix.This publication has 10 references indexed in Scilit:
- THERMAL DEPOLARIZATION OF FLUORESCENCE FROM POLYTENE CHROMOSOMES STAINED WITH ACRIDINE ORANGEThe Journal of cell biology, 1967
- Supercoiling of polyoma virus DNA measured by its interaction with ethidium bromideJournal of Molecular Biology, 1967
- Spectral analysis of the binding of acridine orange to polytene chromosomes.Proceedings of the National Academy of Sciences, 1966
- Changes in deoxyribonucleoprotein during spermiogenesis in the bullExperimental Cell Research, 1966
- Organization of DNA in Dipteran Polytene Chromosomes as Indicated by Polarized Fluorescence MicroscopyScience, 1966
- Intrinsic Birefringence of Multiple-Coiled DNA, Theory and ApplicationsBiophysical Journal, 1965
- THE ARRANGEMENT OF CHROMOSOMES IN THE MATURE SPERM OF THE GRASSHOPPERThe Journal of cell biology, 1964
- Sleep Deprivation, Age, and Exhaustion Time in the RatScience, 1962
- Sleep Deprivation, Age, and Exhaustion Time in the RatScience, 1962
- Crystallinity in sperm heads: Molecular structure of nucleoprotein in vivoBiochimica et Biophysica Acta, 1953