Isolation and Characterization of Somatostatin from Anglerfish Pancreatic Islet*
- 1 December 1979
- journal article
- research article
- Published by The Endocrine Society in Endocrinology
- Vol. 105 (6), 1410-1415
- https://doi.org/10.1210/endo-105-6-1410
Abstract
Somatostatin was purified from anglerfish pancreatic islets using acetic acid extraction, gel filtration (Bio-Gel P-10), ion exchange chromatography (CM Bio-Gel A), and reversed phase high pressure liquid chromatography. The resulting peptide was characterized by RIA, bioassay, and determination of amino acid composition. Anglerfish islet somatostatin was found to possess an amino acid composition and immunological and biological activities equivalent to synthetic somatostatin. Sequence analyses revealed that the primary structure was HAla- Gly-cyclo-[Cys-Lys-Asn-Phe-Phe-Trp-Lys-Thr-Phe-Thr-Ser-Cys]-OH. These results demonstrate that anglerfish islet omatostatin has the same primary structure as somatostatin from all other sources characterized to date.Keywords
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