Simian Virus 40 T Antigen Binds to DNA
- 1 September 1974
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 71 (9), 3754-3757
- https://doi.org/10.1073/pnas.71.9.3754
Abstract
The T antigen of simian virus 40, which may play a role in the control of viral DNA replication, is recovered from nuclei of cells transformed by simian virus 40 in several forms sedimenting at different rates. The large molecular weight forms are converted to the smallest (5 S) form by high salt, suggesting that they differ in the degree of aggregation. All the forms of the antigen bind efficiently to double-stranded DNA-cellulose columns at pH 6.2 and low salt, and elute in two fractions: one at pH 8.0 and low salt, the other at pH 8.0 and high salt. The antigen has little affinity for single-stranded DNA.Keywords
This publication has 13 references indexed in Scilit:
- Polyoma gene function required for viral DNA synthesisVirology, 1973
- Characterization of the SV40 T antigenVirology, 1973
- Studies of the binding of Escherichia coli RNA polymerase to DNA: I. The role of sigma subunit in site selectionJournal of Molecular Biology, 1972
- The transformation of BHK 21 hamster cells by simian virus 40International Journal of Cancer, 1972
- Polyoma T antigen synthesis by temperature-sensitive mutants of polyoma virusVirology, 1972
- Function of gene 32-protein, a new protein essential for the genetic recombination and replication of T4 bacteriophage DNA.1970
- Simian Virus 40-Induced T and Tumor AntigensJournal of Virology, 1969
- Studies with DNA-cellulose Chromatography. I. DNA-binding Proteins from Escherichia coliCold Spring Harbor Symposia on Quantitative Biology, 1968
- SYNTHESIS OF SV40 TUMOR ANTIGEN DURING REPLICATION OF SIMIAN PAPOVAVIRUS (SV40)Proceedings of the National Academy of Sciences, 1964
- A SPECIFIC COMPLEMENT-FIXING ANTIGEN PRESENT IN SV40 TUMOR AND TRANSFORMED CELLSProceedings of the National Academy of Sciences, 1963