Synthesis of the dodecapeptide amide corresponding to the entire amino acid sequence of granuliberin-R, a new frog skin peptide from Rana rugosa.

Abstract

Amino acid sequence of a new frog skin peptide named granuliberin-R [with potent mast cell degranulating activity], H-Phe-Gly-Phe-Leu-Pro-Ile-Tyr-Arg-Arg-Pro-Ala-Ser-NH2, was assessed by 2 conventional ways of synthesis: one by the methanesulfonic acid procedure and the other by the catalytic hydrogenation procedure at the final deprotection steps.