THE BACTERIAL DEGRADATION OF CATECHOL
- 1 May 1965
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 95 (2), 466-474
- https://doi.org/10.1042/bj0950466
Abstract
Two strains of Pseudomonas grown with phenol were used to prepare cell extracts that metabolized catechol with the transient formation of 2-hydroxymuconic semialdehyde. One of these preparations catalyzed the conversion of 1 mole of catechol into 1 mole each of formate and 4-hydroxy-2-oxovalerate. A method for the determination of 4-hydroxy-2-oxovalerate is described, together with some properties of this compound and its 2,4-dinltrophenylhydrazone. Another partially purified cell extract converted 1 mole of 4-hydroxy-2-oxovalerate, formed enzymically from catechol, into 1 mole each of acetaldehyde and pyruvate. The aldolase had a pH optimum of about 8.8, was stimulated by Mg2+ ions and attacked only 1 enantiomer of synthetic 4-hydroxy-2-oxovalerate.Keywords
This publication has 16 references indexed in Scilit:
- A New Metabolic Pathway of CatecholJournal of Biological Chemistry, 1962
- Metapyrocatechase: a New Catechol-cleaving EnzymeJournal of Biological Chemistry, 1961
- New Pathways in the Oxidative Metabolism of Aromatic Compounds by Micro-OrganismsNature, 1960
- The metabolism of C2 compounds in micro-organisms. 1. The incorporation of [2-14C]acetate by Pseudomonas fluorescens, and by a Corynebacterium, grown on ammonium acetateBiochemical Journal, 1958
- Separation of the Lower Fatty Acids (C1 to C10) by High-Voltage Paper ElectrophoresisNature, 1958
- STUDIES ON OXYGENASES - PYROCATECHASE1957
- The determination of lactic acid in microgram quantitiesBiochemical Journal, 1953
- Chemistry of the Oxidative Metabolism of Certain Aromatic Compounds by Micro-OrganismsNature, 1951
- The coupled oxidation of pyruvate with glutathione and cysteineBiochemical Journal, 1951
- A PRESS FOR DISRUPTING BACTERIA AND OTHER MICRO-ORGANISMS1951