Abstract
Starch gel electrophoresis of leaves of diploid and more particularly tetraploid orchard grass (C. glomerata L.) from cultivars as well as natural populations disclosed several anodal stable bands with fast migration and phosphatase activity. Six different phenotypes with 1 band, many others with 3 regular bands or 6 bands (only in the tetraploid individuals), were observed, showing the dimeric structure of the enzymes. Inheritance studies showed 1 polymorphic locus with 6 codominant alleles AcPH l1.00, AcPH l0.95, AcPH l 0.90, AcPH l0.88, AcPH l1.02 and AcPH l1.04 with differences in enzymatic expression. AcPH l1.00 and AcPH l0.90 were found in the 2 ploidy levels. Results from the tetraploid progenies involved tetravalents in meiosis for individuals originating from cultivars but bivalents in the wild plants. The 2 loci AcPH l and GOT l (the latter coding for a glutamate oxaloacetate transaminase) are not linked and apparently are located on different chromosomes.