Identification of denatured double‐stranded DNA by matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry
- 1 January 1995
- journal article
- research article
- Published by Wiley in Rapid Communications in Mass Spectrometry
- Vol. 9 (6), 537-540
- https://doi.org/10.1002/rcm.1290090613
Abstract
We created double‐stranded DNA (dsDNA) by annealing synthetic oligonucleotides and used matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry to measure the difference in molecular weight of the complementary strands after denaturation. Refined sample preparation and deliberate rectilinear construction of a linear mass spectrometer produced a mass resolution exceeding 1000 for single‐stranded DNA. We present methodologies and demonstrate the potential for analysing dsDNA by determining the mass of the complementary strands.Keywords
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