Studies on sulphatases. 24. The use of barium chloranilate in the determination of enzymically liberated sulphate

Abstract

A modification of the chloranilate procedure for determining small amounts (5-80 [mu]g/0. 2 ml) of enzymically liberated inorganic sulfate is described. With the method potassium sulfate has been recovered quantitatively from a number of different enzyme and tissue preparations. Recoveries of sulfate are unaffected by the presence of a number of common anions. On the other hand, interference by cations is rather pronounced. Within certain limits recoveries of sulfate are unaffected by the presence of the assay substrates for carbohydrate sulfatases. The procedure has been compared with the benzidine method in the determination of the release of sulfate from glucose 6-sulfate and chondroitin sulfate by glycosulfatase and chondrosulfatase respectively.