Malonate as a Participant in Organic Acid Metabolism in Bush Bean Leaves.

Abstract

Malonate was shown to be a major constituent of the organic acids of bush bean leaves and was readily metabolized by the particulate fraction of the leaves. The formation of labeled acetate from 2-C14 malonate under anaerobic conditions indicated that acetate was a participant in a pathway of malonate metabolism. The occurrence of labeled Krebs cycle acids suggested the condensation of labeled acetate with OAA to form labeled citrate. The cofactors required for the decarboxylation of malonate were found to be CoA, ATP, GSH, and Mn. The identification of malonyl dihydroxamate indicated the metabolism of malonate proceeded by way of malonyl CoA.