On the mechanism of oligonucleotide-primed RNA synthesis. II. Synthesis of specific primer-initiated RNA copies suitable for DNA sequence analysis
- 1 February 1977
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 4 (2), 445-455
- https://doi.org/10.1093/nar/4.2.445
Abstract
The effect of temperature and primer concentration on oligonucleotide-primed transcription has been studied using the separated strands of a well-defined natural DNA as template. Results were similar to those obtained in the homopolymer-directed model systems. At high temperature and excess primer concentration mainly primer-initiated RNA copies are synthesized. Omission of one ribonucleoside triphosphate also makes the termination specific. The unique RNA fragments thus obtained have been used to determine the perfectly-repeated sequence of 68 base pairs in this DNAKeywords
This publication has 26 references indexed in Scilit:
- Sequence of promoter for coat protein gene of bacteriophage fdNature, 1976
- Bacteriophage T7 early promoters: Nucleotide sequences of two RNA polymerase binding sitesJournal of Molecular Biology, 1975
- Recent Advances in DNA Sequence AnalysiCRC Critical Reviews in Biochemistry, 1975
- Studies on bacteriophage fd DNA. III. Nucleotide sequence preceding the RNA start-site on a promoter-containing fragmentNucleic Acids Research, 1975
- The size of the repeating unit of the repetitive mitochondrial DNA from a “low-density” petite mutant of yeastBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1974
- [22] Preparation of RNA transcripts of discrete segments of DNAMethods in Enzymology, 1974
- Transcription of the early region of bacteriophage T7: Selective initiation with dinucleotidesJournal of Molecular Biology, 1973
- Differential effects of σ factor, ionic strength, and ribonucleoside triphosphate concentration on the transcription of phage T4 DNA with the ribonucleic acid polymerase of Escherichia coliBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1973
- RNA Initiation with Dinucleoside Monophosphates during Transcription of Bacteriophage T4 DNA with RNA Polymerase of Escherichia coliProceedings of the National Academy of Sciences, 1973
- Studies on polynucleotides. CXX. On the transcription of a synthetic 29-unit long deoxyribopolynucleotide.1972