Separation and Determination of 2,5-Dimethoxy-4-Methylamphetamine Enantiomers in Plasma by High-Performance Liquid Chromatography

Abstract

A method for quantitation of 2,5-dimethoxy-4-methylamphetamine (DOM) enantiomers in plasma by high-performance liquid chromatography (HPLC) is described, d- and l-DOM were readily converted to the amides by condensation with a newly developed chiral reagent, succinimidyl ester of l-α-methoxy-α-methyl-1-naphthaleneacetic acid. The yielded diastereomers were separated on the μPorasil column with cyclohexane/ethyl acetate (3:1) exhibiting satisfactory k' and R values. The clean-up procedure by use of Sep-pak C₁₈ and carboxymethyl Sephadex LH-20 (CM-LH-20) proved to be effective for determination of the drug in biological fluids by HPLC. The plasma levels of d- and l-DOM after administration of the racemate to the rabbit were determined by the method thus established.