Properties of salt-resistant lipase and lipoprotein lipase purified from human post-heparin plasma

Abstract

Lipoprotein lipase and salt-resistant lipase were isolated from human post-heparin plasma. The proteins of human post-heparin-plasma lipoprotein lipase and salt-resistant lipase were identified and demonstrated to be immunologically different. Significant differences between the 2 enzymes in their relative amino acid composition were demonstrated, which indicates that the 2 enzymes are different proteins. When analyzed by sodium dodecyl sulfate/polyacrylamide-gel electrophoresis, the enzymes seemed to have monomer MW similar to that of lipoprotein lipase purified from bovine milk.