Cross-Linking of Tobacco Mosaic Virus RNA and Capped Polyribonucleotides to 18S rRNA in Wheat Germ Ribosome-mRNA Complexes1
- 1 December 1983
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 94 (6), 1803-1808
- https://doi.org/10.1093/oxfordjournals.jbchem.a134532
Abstract
Tobacco mosaic virus RNA, forming 40S or 80S initiation complexes with wheat germ ribosomes, was covalently bound to 18S ribosomal RNA by the photoreaction with an RNA cross-linking agent, 4′-aminomethyl-4,5′,8-trimethylpsoralen (AMT). Synthetic polyribonucleotide, poly(A, U), with the cap structure m7GpppGmC at the 5′-terminal was also cross-linked to 18S ribosomal RNA in 40S or 80S complexes with ribosomes by the AMT photoreaction. Polyuridylic acid with the same 5′-cap structure, forming 40S complexes but not 80S complexes with ribosomes, was most efficiently cross-linked to 18S ribosomal RNA by the psoralen photoreaction. These results suggest that the interactions between mRNA and 18S rRNA are not necessarily of strict complementarity but occur during formation of the complexes in eukaryotes. The 40S complexes would be then converted to 80S complexes in the presence of the AUG initiation codon or AUG-like triplets containing A and U on the polyribonucleotide chains which interact with 18S ribosomal RNA.Keywords
This publication has 18 references indexed in Scilit:
- Nonspecific effect of m7GMP on protein-RNA interactions.Journal of Biological Chemistry, 1978
- Influence of 5'-terminal cap structure on the initiation of translation of vaccinia virus mRNA.Journal of Biological Chemistry, 1978
- Nucleotide Sequence at the 5' Extremity of Tobacco-Mosaic-Virus RNA. 1. The Noncoding Region (Nucleotides 1-68)European Journal of Biochemistry, 1978
- Isolation of chicken hemoglobin mRNA and synthesis of complementary DNA.Journal of Biological Chemistry, 1977
- Synthesis and characterization of new psoralen derivatives with superior photoreactivity with DNA and RNABiochemistry, 1977
- Capping of eucaryotic mRNAsCell, 1976
- Differential synthesis of blocked and unblocked 5'-termini in reovirus mRNA: effect of pyrophosphate and pyrophosphatase.Proceedings of the National Academy of Sciences, 1976
- Effect of 5′-terminal structure and base composition on polyribonucleotide binding to ribosomesJournal of Molecular Biology, 1976
- In vitro and in vivo translation of the ribonucleic acids of a cowpea strain of tobacco mosaic virusVirology, 1976
- Messenger RNA for the coat protein of tobacco mosaic virusNature, 1976