Production of Human IgM Anti‐D in Tissue Culture by EB‐Virus‐transformed Lymphocytes
- 1 October 1980
- journal article
- research article
- Published by Wiley in Scandinavian Journal of Immunology
- Vol. 12 (4), 355-358
- https://doi.org/10.1111/j.1365-3083.1980.tb00077.x
Abstract
Lymphocytes obtained from two donors who were producing anti-D were transformed with Epstein-Barr virus and grown in tissue culture. The lymphoblasts from both donors produced anti-D antibody, which agglutinated Rh-positive cells in saline. Both antibodies belonged to the IgM class; the concentration of anti-D in the culture fluid from one of the donors was approximately 250–500 ng/ml and bound firmly to Rh-positive cells with a functional affinity constant of approximately 1 × 109 M−1.This publication has 7 references indexed in Scilit:
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