Murine leukaemogenesis: monoclonal antibodies to T-cell determinants arrest T-lymphoma cell proliferation
- 1 May 1980
- journal article
- letter
- Published by Springer Nature in Nature
- Vol. 285 (5762), 259-261
- https://doi.org/10.1038/285259a0
Abstract
We have proposed a receptor-mediated leukaemogenesis hypothesis wherein T lymphomas would be clones of T cells bearing mitogen-linked surface receptors specific for the envelope determinants of the inducing MuLV1–3. A prediction of the hypothesis is that T-lymphoma proliferation is dependent on continued presentation of MuLV envelope determinants to these cell-surface receptors, and that substances which interfere with receptor–virus interactions should inhibit T-lymphoma proliferation. Rat monoclonal antibodies were raised to the AKR mouse T lymphoma KKT-2, and these antibodies were screened independently for blockade of virus-binding and for cytostatic activity on KKT-2 cells. We report here that those monoclonal antibodies which block virus binding inhibit growth of KKT-2 cells in vitro, whereas monoclonal antibodies which bind to these cells but do not block virus binding are not cytostatic. Three of the four cytostatic antibodies detect determinants on the Thy-1 molecule, while none of the other (non-cytostatic) antibodies detect Thy-1. Antibody inhibition of KKT-2 cell growth is precluded by saturation of KKT-2 virus receptors with the inducing leukaemia virus.Keywords
This publication has 11 references indexed in Scilit:
- Monoclonal antibodies against murine leukemia viruses: Identification of six antigenic determinants on the p15(E) and gp70 envelope proteinsVirology, 1979
- Xenogeneic Monoclonal Antibodies to Mouse Lymphoid Differentiation Antigens*Immunological Reviews, 1979
- Lymphoma development in mice and humans: diversity of initiation is followed by convergent cytogenetic evolution.Proceedings of the National Academy of Sciences, 1979
- AKR leukemogenesis: Identification and biological significance of thymic lymphoma receptors for AKR retrovirusesCell, 1979
- Chromosome Changes (Trisomies #15 and 17) Associated With Tumor Progression in Leukemias Induced by Radiation Leukemia Virus2JNCI Journal of the National Cancer Institute, 1978
- IMMUNOGENETICS OF CELL SURFACE ANTIGENS OF MOUSE LEUKEMIAAnnual Review of Genetics, 1977
- Antibodies to major histocompatibility antigens produced by hybrid cell linesNature, 1977
- Conversion of restrictive mouse cells to permissiveness during sequential and mixed double infection by murine leukemia virusesVirology, 1976
- QUANTITATIVE STUDIES OF NATURALLY OCCURRING MURINE LEUKEMIA VIRUS INFECTION OF AKR MICEThe Journal of Experimental Medicine, 1972
- Selection of Hybrids from Matings of Fibroblasts in vitro and Their Presumed RecombinantsScience, 1964