Somatic cell hybrids producing antibodies specific for the tumor antigen of simian virus 40.

Abstract

Somatic cell hybrids between mouse myeloma cells deficient in hypoxanthine phosphoribosyltransferase (IMP:pyrophosphate phosphoribosyltransferase; EC 2.4.2.8) and spleen cells derived from mice primed with syngeneic or allogeneic cells transformed by SV-40 were produced. Such hybrids produced antibodies specific for SV-40 tumor (T) antigen. Only 4 of 12 independent hybrid cell cultures produced antibodies against SV-40 T antigen that crossreacted with the T antigen induced by BK virus, a human papovavirus isolated from patients who underwent immunosuppressive therapy.