Acetylation of Choline and Homocholine by Membrane‐Bound Choline‐O‐Acetyltransferase in Mouse Forebrain Nerve Endings
- 1 February 1981
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 36 (2), 732-740
- https://doi.org/10.1111/j.1471-4159.1981.tb01649.x
Abstract
The choline analog homocholine is not acetylated in vitro by choline-O-acetyltransferase (ChAT, EC 2.3.1.6), which is solubilized by 100 mM-sodium phosphate buffer washes of a crude vesicular fraction of mouse forebrain. However, both homocholine and choline are acetylated by a form of ChAT which is nonionically associated with a subcellular fraction of mouse forebrain containing membrane-associated organelles and occluded acetylcho-line (P4). Acetylation of homocholine by membrane-associated ChAT is saturable. 4-(1-Naphthylvinyl)pyridine (NVP) inhibits the acetylation of both choline (60%) and homocholine (40%) by membrane-associated ChAT but reduces the acetylation of choline alone by soluble ChAT (76%). Choline and homocholine serve as competitive alternative substrates for the same membrane-associated ChAT, whereas homocholine acts only as a competitive inhibitor of choline acetylation by soluble ChAT. Acetylhomocholine competitively inhibits the acetylation of choline by both soluble and membrane-associated ChAT more dramatically than does the natural end product, acetylcholine.Keywords
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