The structure of the polysaccharide moiety of Rhodopseudomonas sphaeroides ATCC 17023 lipopolysaccharide
Open Access
- 1 October 1984
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 144 (2), 227-232
- https://doi.org/10.1111/j.1432-1033.1984.tb08454.x
Abstract
The chemical structure of the polysaccharide moiety of the lipopolysaccharide of Rhodopseudomonas sphaeroides ATCC 17023 was established. Mild acetic acid hydrolysis of isolated lipopolysaccharide, followed by preparative high-voltage paper electrophoresis afforded three oligosaccharides. They were characterized by chemical and physicochemical studies to be: GlcA(α1→4)dOclA8P, Thr(6′) GlcA(α1→4)GlcA and GlcA(α1→4)dOclA, where GlcA is d-glucuronic acid and dOclA is 3-deoxy-d-manno-octulosonic acid. Carboxyl-reduction of the lipopolysaccharide followed by acid hydrolysis gave a trisaccharide: GlcA(α1→4)Glc(α1→4)G1c, showing the presence of three residues of glucuronic acids in the O-specific chain and indicating that only two of them are reducible by NaBH4. The linkage between the polysaccharide and lipid A was shown to be through a single 1,4-linked residue of dOclA attached by a 2,6′-linkage to the lipid A moiety.This publication has 30 references indexed in Scilit:
- Structural studies on the non‐toxic lipid A from Rhodopseudomonas sphaeroides ATCC 17023European Journal of Biochemistry, 1983
- Cell‐Wall Lipopolysaccharide of Escherichia coli O114:H2European Journal of Biochemistry, 1983
- Differential Determination of the 3‐Deoxy‐d‐mannooctulosonic Acid Residues in Lipopolysaccharides of Salmonella minnesota Rough MutantsEuropean Journal of Biochemistry, 1983
- Somatic Antigens of Pseudomonas aeruginosaEuropean Journal of Biochemistry, 1982
- Somatic Antigens of Pseudomonas aeruginosaEuropean Journal of Biochemistry, 1982
- Lipopolysaccharide Heterogeneity in Salmonella typhimurium Analyzed by Sodium Dodecyl Sulfate/Polyacrylamide Gel ElectrophoresisEuropean Journal of Biochemistry, 1980
- Lipopolysaccharides of Photosynthetic ProkaryotesAnnual Review of Microbiology, 1979
- The Linkage of Lysine in the O‐Specific Chains of Proteus mirabilis 1959European Journal of Biochemistry, 1976
- Thiobarbituric Acid Spray Reagent for Deoxy Sugars and Sialic AcidsNature, 1960
- Detection of Sugars on Paper ChromatogramsNature, 1950