Effects of adenosine 3′:5′-cyclic monophosphate and serum on synthesis of hyaluronic acid in confluent rat fibroblasts
- 15 March 1977
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 162 (3), 539-543
- https://doi.org/10.1042/bj1620539
Abstract
A small amount of hyaluronic acid is synthesized in confluent cultures of rat fibroblasts, which have a high content of cyclic AMP. Addition of calf serum caused a rapid decrease in the cellular cyclic AMP content and large increases in hyaluronic acid synthetase activity and hyaluronic acid production. Addition of cyclic AMP also caused a marked increase in hyaluronic acid synthetase activity within 2h and then increased hyaluronic acid production. The effects of cyclic AMP and serum on hyaluronic acid synthesis were additive. Prostaglandin E2, which increased the cyclic AMP by stimulating adenylate cyclase, was as effective as cyclic AMP in increasing hyaluronic acid synthetase activity, but AMP was far less effective than cyclic AMP. These results indicate that cyclic AMP itself stimulates the mucopolysaccharide synthesis and that the effect of serum is not due to a decrease in cyclic AMP in the cells.This publication has 18 references indexed in Scilit:
- The enhanced production of hyaluronic acid by cultured rat fibroblast cells treated with cyclic AMP and its dibutyryl derivativeJournal of Cellular Physiology, 1976
- Pleiotypic Control by Adenosine 3′:5′-Cyclic Monophosphate: A Model for Growth Control in Animal CellsProceedings of the National Academy of Sciences, 1973
- Regulation of cell growth by cyclic adenosine 3',5'-monophosphate. Effect of cell density and agents which alter cell growth on cyclic adenosine 3',5'-monophosphate levels in fibroblasts.1972
- Growth Control and Cyclic Alterations of Cyclic AMP in the Cell CycleNature New Biology, 1972
- Opposite effects of dibutyryl adenosine 3′:5′ cyclic monophosphate and serum on growth of Chinese hamster cellsJournal of Cellular Physiology, 1972
- The Effect of Dibutyryl Cyclic Adenosine Monophosphate on Synthesis of Sulfated Acid Mucopolysaccharides by Transformed FibroblastsJournal of Biological Chemistry, 1972
- Prostaglandin E 1 Effects on Adenosine 3′:5′-Cyclic Monophosphate Concentration and Phosphodiesterase Activity in FibroblastsProceedings of the National Academy of Sciences, 1972
- A Protein Binding Assay for Adenosine 3′:5′-Cyclic MonophosphateProceedings of the National Academy of Sciences, 1970
- Comparative biochemistry of skin the mucopolysaccharides of shark skinBiochimica et Biophysica Acta, 1963
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951