Effect of Plasma from Bled and Phenylhydrazine Treated Animals on Plasm Iron Turnover. A Test for "Hemopoietine"

Abstract

Plasma obtained from phenylhydrazine treated rabbits markedly increases plasma Fe turnover, when injected intraperitoneally into normal rabbits, in doses of 10 ml/kg twice a day for two days. The supernatant obtained after boiling this plasma at pH 5.5 retains its effect on plasma Fe turnover. Plasma from repeatedly bled rabbits, does not affect plasma Fe turnover, but produces a marked drop in plasma Fe concentration. Plasma Fe turnover is increased however by dialyzed plasma from these rabbits. Acid acetone extracts of bled rabbits" plasma depresses both plasma Fe concentration and turnover. It is suggested that plasma from both bled and phenylhydrazine treated rabbits, contains a factor stimulating hemoglobin synthesis (Hemopoietine) and that plasma from severely bled rabbits contains as well a factor depressing both plasma Fe concentration and turnover.