A Method of Permanently Mounting Biological Tissue Cleared in Herr's Four-And-A-Half Clearing Fluid
- 1 January 1976
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 51 (2), 125-130
- https://doi.org/10.3109/10520297609116683
Abstract
A technique which should be generally applicable for preparing permanent mounts of tissue cleared in Herr's four-and-a-half clearing fluid is described. This technique involves transferring plant or animal tissues through a series of solutions consisting of Pienarr's fixative, Herr's clearing fluid, chloral hydrate, acetone and finally polyester resin for mounting. Material prepared using this method is exceptionally transparent and well preserved, and is suitable for either phase contrast or Nomarski interference microscopy.This publication has 7 references indexed in Scilit:
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