The analysis of hair keratin

Abstract

Conditions for the quantitative isolation of proline, suitable for use in a systematic scheme of separation of amino-acids from hydrolyzed hair keratin, have been studied. Maximum yield of proline (4.3%) was obtained by application of Town''s copper salt method, after preliminary removal of cystine, arginine, most of the tyrosine, the dicarboxylic acids, histidine, lysine and some leucine had been effected. No hydroxyproline could be found in human hair hy-drolysates.