Transformation in Escherichia coli: cryogenic preservation of competent cells
- 1 October 1977
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 132 (1), 349-351
- https://doi.org/10.1128/jb.132.1.349-351.1977
Abstract
E. coli cells prepared for transformation by treatment with cold 0.1 M CaCl2 remained viable and competent after storage at -82.degree. C in 15% glycerol; thawed-cell samples yielded up to 106 transformants/.mu.g of plasmid DNA.This publication has 13 references indexed in Scilit:
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