Functional characterization of the H-2Dp gene product

Abstract

A λ clone containing the D ^p gene was used to transform L cells. The D^p product expressed was identified by two-dimensional gel electrophoreis and flow cytometry. The D^p product expressed by the L cells was recognized by D^P-specific flow cytometry. The D^p product expressed by the L cells was recognized by D^p-specific but not K^p-specific killer T cells. This killing was inhibited by monoclonal antibodies specific for D^p but not K^p or K^k antigens. Similarly, lymphocytic choriomeningitis virus (LCMV) killer T cells from B 10.P mice were able to kill LCMV-infected L12a cells, but not LCMV-infected Ltk⁺. Again only D^p monoclonal antibodies could inhibit this killing.