All VH11 genes expressed in peritoneal lymphocytes encode anti‐bromelain‐treated mouse red blood cell autoantibodies but other VH gene families contribute to this specificity
- 1 July 1990
- journal article
- research article
- Published by Wiley in European Journal of Immunology
- Vol. 20 (7), 1583-1589
- https://doi.org/10.1002/eji.1830200726
Abstract
We have studied the relationship between B cell reactivity to bromelain‐treated autologous mouse erythrocytes (BrMRBC) and expression of the VH11 gene family in splenic, peritoneal and pleuropericardial cell populations from normal C57BL/6 mice. B lymphocytes producing antibodies to BrMRBC were selectively enriched or depleted from normal populations by rosette formation with BrMRBC, followed by centrifugation over density gradients. This selection method, based on the presence of functional receptors (membrane IgM), is harmless for the cells and allowed subsequent cloning in agar (colony‐forming unit‐B). The utilization of the 10 VH gene families was then scored in mRNA colony blot assays. The analysis of > 650 anti‐BrMRBC clones and > 350 VH11‐expressing colonies indicates that about half of those antibody reactivities are encoded by VH11 genes. Furthermore, it appears that all VH11‐expressing B cells in the peritoneal cavity produce anti‐BrMRBC antibodies.This publication has 56 references indexed in Scilit:
- Immunocompetence of peritoneal B cellsResearch in Immunology, 1989
- Structural Correlates of a Regulatory IdiotopeImmunological Reviews, 1989
- Biased immunoglobulin variable region gene expression by Ly‐1 B cells due to clonal selectionEuropean Journal of Immunology, 1989
- Ontogenic development of autoantibody repertoires in spleen and peritoneal cavity of normal mice: examples of T cell-dependent and -independent reactivitiesEuropean Journal of Immunology, 1989
- Expression of VH11-gene family in hybridoma collections from peritoneum and spleen: Differential correlation with BrMRBC reactivityResearch in Immunology, 1989
- Phosphatidyl choline is recognized by a series of Ly-1+ murine B cell lymphomas specific for erythrocyte membranes.The Journal of Experimental Medicine, 1986
- Preferential utilization of the most JH-proximal VH gene segments in pre-B-cell linesNature, 1984
- Multiple VH gene segments encode murine antistreptococcal antibodies.The Journal of Experimental Medicine, 1984
- LPS-induced autoantibody response: I. Ontogenic development of PFC response to bromelain-treated syngeneic erythrocytesCellular Immunology, 1980
- Studies on antigen-binding cells: II. Relationship to antigen-sensitive cellsCellular Immunology, 1970