Effect of Ca2+ ions on plasmid transformation of Streptococcus lactis protoplasts
- 1 October 1985
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 50 (4), 1100-1102
- https://doi.org/10.1128/aem.50.4.1100-1102.1985
Abstract
The effects of Mg2+ and Ca2+ ions on the efficiency of the plasmid transformation of lysozyme-treated Streptococcus lactis protoplasts were compared. A 33-megadalton plasmid, pLP712, coding for lactose fermentation and a 6.5-megadalton plasmid, pGB301, coding for erythromycin and chloramphenicol resistance were used as model plasmids, and S. lactis MG1614 was the recipient. Replacing Mg2+ with Ca2+ in the transformation buffer was found to increase transformant frequency more than 10-fold with both plasmids.Keywords
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