IDENTIFICATION OF GENETIC-VARIANTS IN ERYTHROCYTE LYSATE BY TWO-DIMENSIONAL GEL-ELECTROPHORESIS
- 1 January 1984
- journal article
- research article
- Vol. 36 (3), 601-612
Abstract
Two-dimensional gel electrophoresis followed by Ag-staining was employed to study 27 red cell lysates for genetic variation. Polypeptides (46) selected without respect to variability were considered suitable for scoring. Only 23 of the total of 1242 polypeptides could not be scored unambiguously. Of the remaining 1219 polypeptides, 38 exhibited the combination of a normal and a variant polypeptide. All variants were present in either the father or the mother of the subjects. The observed index of heterozygosity was 3.1% .+-. 0.5%.This publication has 23 references indexed in Scilit:
- Two-dimensional electrophoresis of plasma polypeptides reveals "high" heterozygosity indices.Proceedings of the National Academy of Sciences, 1983
- Human hypoxanthine (guanine) phosphoribosyltransferase: an amino acid substitution in a mutant form of the enzyme isolated from a patient with gout.Proceedings of the National Academy of Sciences, 1983
- Inhibition of Fertility in Rabbits by Monoclonal Antibodies Against SpermBiology of Reproduction, 1983
- Genetic analysis of human lymphocyte proteins by two-dimensional gel electrophoresis: 4. Genetic polymorphism of cytosol 100k polypeptideHuman Genetics, 1982
- Genetic analysis of human lymphocyte proteins by two-dimensional gel electrophoresis: 3. Frequent occurrence of genetic variants in some abundant polypeptides of PHA-stimulated peripheral blood lymphocytesHuman Genetics, 1982
- Genetic analysis of human lymphocyte proteins by two-dimensional gel electrophoresis: 2. Genetic polymorphism of lymphocyte cytosol 64K polypeptideHuman Genetics, 1982
- Genetic analysis of human lymphocyte proteins by two-dimensional gel electrophoresis:Human Genetics, 1981
- Human heterozygosity: a new estimate.Proceedings of the National Academy of Sciences, 1979
- Microheterogeneity of serum transferrin, haptoglobin and α2HS glycoprotein examined by high resolution two-dimensional electrophoresisBiochemical and Biophysical Research Communications, 1979
- The distribution of isoelectric points of human soluble proteins and in particular of the enzyme products of 88 human gene lociAnnals of Human Genetics, 1977