Homologous radioimmunoassay for human mid-regional parathyroid hormone

Abstract

A radioimmunoassay, selective for the clinically important mid region of human parathyroid hormone (hPTH), is reported. The synthetic44–68 amino acid sequence (h44–68PTH) was used as both the standard and tracer material. This eliminated many of the undesirable characteristics associated with PTH assays that employ hormone of biological origin. These components allowed the detection of mid regional fragments present in both intact and fragmented hPTH, with a working range between 50 and 2500 pg/ml h44–68PTH. There was no interference from serum proteins and no significant cross reactivity to a range of N-terminal, C-terminal and other mid regional hPTH peptides. The assay proved to be rapid (total time 24 h) and was extremely reproducible, with an intraassay and interassay variation of 2.8% and 5.6% respectively (expressed as percentage SE of mean). The plasma concentration of normal subjects was established as 129 ± 6 pg/ml (h44–68PTH) with a range of 50–300 pg/ml (n = 42). This assay, using fully synthetic hPTH peptides, was able to distinguish between euparathyroid and hyperparathyroid subjects, which suggests that the assay is of diagnostic value.