The Fertility of Bovine Semen in Citrate-Yolk Extenders Containing Added Catalase

Abstract
Using the split sample technic, spermatozoan livability and fertility of 47 semen samples from 24 Holstein bulls were studied. The samples were extended to contain approx. 11.9 x 106 motile spermatozoa/ml, in citrate-yolk and citrate-sulfanilamide-yolk extenders, with and without added catalase. On the basis of the avg. % 60- to 90-day non-returns to service in approx. 1700 1st and 2d service cows/treatment, the extenders compared as follows: 3.6 citrate-yolk without added catalase, 60.7; 3.6 citrate-sulfanilamide-yolk without added catalase, 62.2; and 3.6 citrate-sulfanilamide-yolk with added catalase, 61.7. Differences in spermatozoan livability and fertility in the various extenders were not significant.