Vitamins A and D of Spinach

Abstract

An acetone-ether extract of dried spring spinach, more than 200 times as concentrated as the fresh leaves and practically free from mineral matter and oxidizing enzymes, was fed to rats as a source of vitamin A. In doses of 25 mgm. daily it secured freedom from xerophthalmia and induced steady though subnormal growth. This diet, however, did not appear to contain significant amounts of vitamin D as judged by microscopic examination of the bones, the A/R ratio and changes in the fecal [rho]h. A table summarizing the results obtained by other workers on the vitamin A and D content of spinach leaves is given.