The intracellular redistribution of catalase during the incubation of mouse-liver slices

Abstract

During incubation of liver slices at 38[degree] in phosphate saline, catalase disappears from the large granules and art equal amount appears in the extra participate cytoplasm. When granule preparations are incubated in phosphate saline no catalase is liberated. Incubation in 0.25 [image] sucrose results in a transfer of catalase to the medium. No evidence has been found that the granules become lysed or more fragile during incubation. It is concluded that during incubation of liver slices in phosphate saline, catalase migrates from the large granules into the extra particulate cytoplasm, probably through the intact granule membrane, and that a similar process occurs when isolated granules are incubated in 0.25 [image]_ sucrose.