Isolation and Characterization of PDE-I and II, the Inhibitors of Cyclic Adenosine-3′,5′-monophosphate Phosphodiesterase

Abstract

In the screening for inhibitors of cyclic AMP phosphodiesterase, 2 compounds, PDE-I (C13H13N3O5) and PDE-II (C14H14N2O5), were isolated from culture filtrates of a Streptomyces. Concentrations for 50% inhibitions of PDE-I and PDE-II against the high Km enzyme were 15 .mu.M and 13 .mu.M, and those against the low Km enzyme were 65 .mu.M and 130 .mu.M, respectively. Production, isolation and characterization of these compounds are described.