Kinetic parameters from progress curves of competing substrates. Application to β-lactamases
- 1 May 1983
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 211 (2), 511-513
- https://doi.org/10.1042/bj2110511
Abstract
The use of 2 substrates, one of them a reporter substrate, is often convenient. The time course of an enzyme reaction with competing substrates is given explicitly. Kinetic parameters can be readily obtained. The method is applied to the hydrolysis of benzylpenicillin, with cephalosporin C as the reporter substrate, catalyzed by a Pseudomonas .beta.-lactamase.This publication has 11 references indexed in Scilit:
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